General Cell Based Assays
Eurofins Discoverx PathHunter eXpress PTGER2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0214E2CP2L)
The PathHunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER2 and EA-tagged β-Arrestin-2. Activation of PTGER2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Assay Kit suffici
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis to fatty acids or energy through acetyl-CoA to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.
Meso Scale Discovery R-PLEX Mouse Pentraxin 3 ASY
The Meso Scale Discovery R-PLEX Mouse Pentraxin 3 Assay is a multiplex immunoassay that quantitatively measures mouse Pentraxin 3 in serum plasma and cell culture supernatants This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The assay requires 50 L or less sample volume and can perform up to 80 tests across two plates The assay has a lower limit of detection of 0 04 ng/mL and an upper limit of 10 000 ng/mL This assay allows for the rapid reproducible quantification of mouse Pentraxin 3 from various biological matrices
Eurofins Discoverx PathHunter eXpress GCGR Total Internalization Assay, 2pl Kit. (93-0820E1CP0M)
The PathHunter GCGR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GCGR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged GCGR and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GCGR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-FOXO1 (Ser256) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-FOXO1 (Ser256) Detection Kit, 10,000 Assay Points
Sigma Aldrich Fine Chemicals Biosciences 7-Aminoactinomycin D ~97% (HPLC), powder | 7240-37-1 | MFCD00077295 | 5MG
7-Aminoactinomycin D ~97% (HPLC), powder | Purity: ~97% (HPLC) | Mol Wt: 1270.43 | 7240-37-1 | MFCD00077295 | 5MG
Revvity Health Sciences Inc HTRF Human Pro-Collagen Type 1 Detection Kit, 500 Assay Points
HTRF Human Pro-Collagen Type 1 Detection Kit, 500 Assay Points
Eurofins Discoverx cAMP Hunter eXpress PROKR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0090E2CP2L)
The cAMP Hunter PROKR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PROKR1, a naturally Gs coupled GPCR. Upon activation of PROKR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EpoR-JAK2 Functional Assay, 2-Plate Kit. (93-0900E3CP16M)
The PathHunter EpoR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses EpoR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EpoR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mPTGER4 DLD1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0982E13MCP25L)
The PathHunter mPTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of mPTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTGER4 and EA-tagged β-Arrestin-2. Activation of mPTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TBXA2R U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0322E3CP5M)
The PathHunter TBXA2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. thromboxane A2, prostaglandins) based activation of TBXA2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TBXA2R and EA-tagged β-Arrestin-2. Activation of TBXA2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences FLUOR DE LYS HDAC fluorometric cellular activity assay kit (1x96wells)
Monitor HDAC activity in cell culture using cell-permeable FLUOR DE LYS substrate. Alternative Name: Cellular Histone deacetylase fluorescent assay kit. Applications: Fluorescent detection, HTS, Cell-based assays, Activity assay. Contents: Nuclear Extracts from Hela Cells (human cervical cancer cell line) (BML-KI345), FLUOR DE LYS Substrate (BML-KI104), FLUOR DE LYS Developer Concentrate (20x), Trichostatin A (HDAC Inhibitor) (BML-GR-309-9090), Nicotinamide (Sirtuin Inhibitor) (BML-KI283), FLUOR DE LYS Deacetylated Standard (BML-KI142), HDAC Assay Buffer (BML-KI143), Cell Lysis Buffer (2), 1/2 volume microplates (BML-KI101).
Abcam NAD/NADH Assay Kit (Colorimetric)
NAD/NADH Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter ProLabel Detection Kit, 10,000 dp (384-well). (93-0180L)
The PathHunter ProLabel Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 25 plates (384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0180l.
Cellular Dynamics International iCell Microglia Media Kit, 50 ml iCell Glial Base Medium, 0.5 ml iCell Microglia Supplement A (100x), 0.5 ml iCell Microglia Supplement B (100x), 1 ml iCell Neural Supplement C (50X)
The iCell Microglia Media Kit is a specialized cell culture medium tailored for the optimal growth and maintenance of human iPSC-derived microglia. It provides a consistent in vitro environment, essential for research in microglial biology and neurodegenerative diseases. This media kit solves the challenges faced with primary human microglia by ensuring reproducible results for various experiments related to neuroinflammation and drug discovery.
- Supports growth of iPSC-derived human microglia.
- Includes multiple supplements for enhanced performance.
- Stores complete medium at 4°C for up to 2 weeks.
- Can be aliquoted for single-use storage at -20°C for up to 3 months.
- Recommended culture conditions: 37°C and 5% CO2.
- Designed for research purposes only, focusing on neurodegeneration and drug discovery.
- Maintains relevant microglial markers and physiological functions.